| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.122947 |
| Chromosome: | chromosome 16 |
| Location: | 230255 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g694704 | (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAAGGTATGTGGTTGTGTATCCGGGGCCC |
| Internal bar code: | GGGGAGCTAACGGGGTCAAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 844 |
| LEAP-Seq percent confirming: | 77.9233 |
| LEAP-Seq n confirming: | 833 |
| LEAP-Seq n nonconfirming: | 236 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGGTTCACTCCAGTTGGG |
| Suggested primer 2: | TTCCAGGAGTTCCCTCATTG |