| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.122956 |
| Chromosome: | chromosome 17 |
| Location: | 6984884 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g746247 | (1 of 1) K11430 - histone-lysine N-methyltransferase EZH2 (EZH2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGCGCAAAGAGCGAGGAGCTAGAGGCGG |
| Internal bar code: | GGGCCTACGTCTGCTAAAAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 372 |
| LEAP-Seq percent confirming: | 90.8382 |
| LEAP-Seq n confirming: | 16548 |
| LEAP-Seq n nonconfirming: | 1669 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTCTGTCAAGGGGTCAGG |
| Suggested primer 2: | TACGAGGTGTCACTGGTGGA |