| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.122963 |
| Chromosome: | chromosome 10 |
| Location: | 4792306 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g453807 | CTPB1 | (1 of 2) PTHR32060:SF5 - PEPTIDASE S41 FAMILY PROTEIN; C-terminal peptidase B | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCGGATTTGCCACACTTTCACTCGCGGC |
| Internal bar code: | TAAAAGTTCGTCCGGTAAAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 113 |
| LEAP-Seq percent confirming: | 62.6506 |
| LEAP-Seq n confirming: | 104 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAACTGACCTCTGCCGCTC |
| Suggested primer 2: | GAGGGGTGTGTGTGTGTGAG |