| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.122970 |
| Chromosome: | chromosome 6 |
| Location: | 6964584 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g296350 | SENP5,ULP1 | ULP2-type SUMO protease; (1 of 5) 3.4.22.68 - Ulp1 peptidase / Ulp1 protease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGATTATTCCTCAACCAAAGCTGTCTCG |
| Internal bar code: | GATTCGCTCCCGCGTAGTCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 293 |
| LEAP-Seq percent confirming: | 99.4226 |
| LEAP-Seq n confirming: | 7404 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTAGGAGAAGCAGAGGGC |
| Suggested primer 2: | ACTGCTGTTCCCCATCAATC |