Insertion junction: LMJ.RY0402.122970_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.122970
Chromosome:chromosome 6
Location:6964588
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g296350 SENP5,ULP1 ULP2-type SUMO protease; (1 of 5) 3.4.22.68 - Ulp1 peptidase / Ulp1 protease intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCATCACTTCTTGCTGTTTCTCCACCTGTT
Internal bar code:CAGGGACATGCTGATTGCGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:58
LEAP-Seq percent confirming:99.6951
LEAP-Seq n confirming:327
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTAGGAGAAGCAGAGGGC
Suggested primer 2:ACTGCTGTTCCCCATCAATC