| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.122986 |
| Chromosome: | chromosome 2 |
| Location: | 5979070 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g112150 | (1 of 10) 3.1.1.5 - Lysophospholipase / Phospholipase B | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGACTAGTGGTACCGGGTGATGGAGGGCG |
| Internal bar code: | TCTGAATGTGGGGGGACGCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 825 |
| LEAP-Seq percent confirming: | 99.9038 |
| LEAP-Seq n confirming: | 2076 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACAACCTGACCGCCTATCC |
| Suggested primer 2: | GGGGCAGTGACATACGAACT |