| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.123007 |
| Chromosome: | chromosome 17 |
| Location: | 3166916 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g721800 | (1 of 4) IPR009019 - K homology domain, prokaryotic type | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCCTGCGCTGTTGGTGCCACCACTGCGT |
| Internal bar code: | CGGCCCGTATTGCCGAATATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 260 |
| LEAP-Seq percent confirming: | 99.6312 |
| LEAP-Seq n confirming: | 1891 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACACCGAGTAAGCGACTG |
| Suggested primer 2: | GTGCCCTACAAGTCCATCGT |