Insertion junction: LMJ.RY0402.123019_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.123019
Chromosome:chromosome 17
Location:1409082
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g706450 LIC3 (1 of 1) IPR000104//IPR006029//IPR006201//IPR006202 - Antifreeze protein, type I // Neurotransmitter-gated ion-channel transmembrane domain // Neurotransmitter-gated ion-channel // Neurotransmitter-gated ion-channel ligand-binding domain; Ligand-gated ion channel intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAGCCCAAGCTAAACCAATCCAAGCTGGC
Internal bar code:CATGTTCATTTGGGGGGGGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:16
LEAP-Seq percent confirming:95.9184
LEAP-Seq n confirming:47
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTCTCCCTACCCCTCTCA
Suggested primer 2:GGTATCGTGTTGACGTCGTG