Insertion junction: LMJ.RY0402.123028_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.123028
Chromosome:chromosome 16
Location:137873
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g695250 (1 of 726) IPR011009 - Protein kinase-like domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGTTGTTTAGGGTTACAACCCCATTATT
Internal bar code:CCACACGATCGTGTGCCCGTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:711
LEAP-Seq percent confirming:99.5928
LEAP-Seq n confirming:1712
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTGTCGCCACATCGATAC
Suggested primer 2:ACATCATGTTGCTGCTACCG