| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.123034 |
| Chromosome: | chromosome 11 |
| Location: | 3044637 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g478800 | (1 of 1) PTHR31027//PTHR31027:SF2 - FAMILY NOT NAMED // PROTEIN F18H3.4 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAGCCGGAGGCGCCCGCCGCCGCCAAGG |
| Internal bar code: | GTAATCGCACACGGATGTTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 470 |
| LEAP-Seq percent confirming: | 97.2033 |
| LEAP-Seq n confirming: | 1286 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGGTGGACGAGATGTTC |
| Suggested primer 2: | ACCTTGAGCTTCACGTCCAC |