| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.123070 |
| Chromosome: | chromosome 14 |
| Location: | 2929231 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g627576 | DHC7 | (1 of 2) PTHR10676//PTHR10676:SF136 - DYNEIN HEAVY CHAIN FAMILY PROTEIN // DYNEIN HEAVY CHAIN 6, AXONEMAL; Axonemal Dynein Heavy Chain 7 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGGGTAAGCGGCATGTTCAGTTATTAAG |
| Internal bar code: | GATTATAGTCCTGTGTGGGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 443 |
| LEAP-Seq percent confirming: | 92.2303 |
| LEAP-Seq n confirming: | 2291 |
| LEAP-Seq n nonconfirming: | 193 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAGCTAGGAACACCCAAC |
| Suggested primer 2: | CCAGCCCAAACTAAACCAAA |