Insertion junction: LMJ.RY0402.123093_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.123093
Chromosome:chromosome 9
Location:767174
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g402200 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGATGATGCATTAGACATCCTGAGCGCG
Internal bar code:CGACTCAAGTTGCCGTGCGTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:378
LEAP-Seq percent confirming:99.3714
LEAP-Seq n confirming:1739
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TATCCACATATAACGCGCCA
Suggested primer 2:ATGTTGGCTTCCGCATTTAC