Insertion junction: LMJ.RY0402.123151_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.123151
Chromosome:chromosome 4
Location:353729
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre04.g217911 (1 of 752) IPR027417 - P-loop containing nucleoside triphosphate hydrolase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGGCCTGCTGGCGAGCTTCAATCCGCGC
Internal bar code:ATTCCCGACCGTCTGGAGTCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:958
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1625
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:TCGAGATGCAGTACCTCGTG
Suggested primer 2:AAGCCTCGTAGGCGTGTAAA