Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.123157 |
Chromosome: | chromosome 3 |
Location: | 5107967 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g181576 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAACATACCGGTAGACCGACATGGACGGT |
Internal bar code: | TAGACCCGAGGTATTCCCCGCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 796 |
LEAP-Seq percent confirming: | 99.5519 |
LEAP-Seq n confirming: | 4443 |
LEAP-Seq n nonconfirming: | 20 |
LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTTTTGGGACCTTCTGCATC |
Suggested primer 2: | GATGCGAGTGTGGTATGTCG |