| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.123182 |
| Chromosome: | chromosome 9 |
| Location: | 5673738 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401330 | (1 of 1) 3.1.3.21 - Glycerol-1-phosphatase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTACCACCGCCGCGGCGTCCTCTTGATT |
| Internal bar code: | AGATATCCCGGCGCGTGCTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 958 |
| LEAP-Seq percent confirming: | 99.8291 |
| LEAP-Seq n confirming: | 9931 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTATGCATGCTCAGAGTGGC |
| Suggested primer 2: | TGGGAACTGTGCTCTCACTG |