| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.123206 |
| Chromosome: | chromosome 6 |
| Location: | 6790530 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g294950 | ENR1 | (1 of 1) 1.3.1.9 - Enoyl-[acyl-carrier-protein] reductase (NADH) / NADH-specific enoyl-ACP reductase; Enoyl-ACP reductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCGAGGAGCAGGAGAGCGGGAAGTAGGG |
| Internal bar code: | TTGTATTACCAAGGAAGGAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 723 |
| LEAP-Seq percent confirming: | 99.8344 |
| LEAP-Seq n confirming: | 3014 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAACTGGTGTGGTGACGTT |
| Suggested primer 2: | GGGACATGGTGGTTTACAGG |