| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.123206 |
| Chromosome: | chromosome 6 |
| Location: | 6790536 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g294950 | ENR1 | (1 of 1) 1.3.1.9 - Enoyl-[acyl-carrier-protein] reductase (NADH) / NADH-specific enoyl-ACP reductase; Enoyl-ACP reductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTTCCCCTCCCTGCCCTTTGATTGCTAC |
| Internal bar code: | GTCTGCGGGGTGATACATGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 57 |
| LEAP-Seq percent confirming: | 91.3333 |
| LEAP-Seq n confirming: | 274 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGACATGGTGGTTTACAGG |
| Suggested primer 2: | TCAACTGGTGTGGTGACGTT |