| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.123207 |
| Chromosome: | chromosome 7 |
| Location: | 540319 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g316200 | (1 of 726) IPR011009 - Protein kinase-like domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGAGCAAGAGTTGCAAGCATGTACTAGC |
| Internal bar code: | CCAAGAGGCTTAATGCGTTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 499 |
| LEAP-Seq percent confirming: | 99.6935 |
| LEAP-Seq n confirming: | 5205 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTCTTCTGCATCTTGGGC |
| Suggested primer 2: | ACATTCACGCCGAAACCTAC |