| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.123230 |
| Chromosome: | chromosome 16 |
| Location: | 3581975 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g682013 | (1 of 2) PTHR30545:SF2 - SUGAR FERMENTATION STIMULATION PROTEIN A | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATCTGAATCCTCACGTTCTCCACGTCAC |
| Internal bar code: | CAATCCTCCAGCTGGCTCGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 856 |
| LEAP-Seq percent confirming: | 81.5741 |
| LEAP-Seq n confirming: | 1337 |
| LEAP-Seq n nonconfirming: | 302 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGGTGAGGTGTGGTGTCA |
| Suggested primer 2: | TACACGTCAGATGGTCCCAA |