| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.123234 |
| Chromosome: | chromosome 13 |
| Location: | 2595068 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g581200 | (1 of 1) PTHR12241//PTHR12241:SF1 - TUBULIN TYROSINE LIGASE-RELATED // TUBULIN--TYROSINE LIGASE PBY1-RELATED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGTGTCGCAGGATGGCCGACACCAGCGT |
| Internal bar code: | GTTTTCGTGGGGCAGCATGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 924 |
| LEAP-Seq percent confirming: | 99.906 |
| LEAP-Seq n confirming: | 3189 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGAACACACCTCAGCAAA |
| Suggested primer 2: | TTTAGCGGTCTGCACGTATG |