| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.123240 |
| Chromosome: | chromosome 1 |
| Location: | 5551115 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g039400 | (1 of 1) 3.8.1.5 - Haloalkane dehalogenase / 1-chlorohexane halidohydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGTGGAGTGACGGGTCTGAGATTTGAGC |
| Internal bar code: | CGCTCATACGAGACTCCACAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1258 |
| LEAP-Seq percent confirming: | 97.731 |
| LEAP-Seq n confirming: | 603 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGCCTGTTATTCAGTGCG |
| Suggested primer 2: | TTGAGGTTCCCAGGTATTCG |