| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.123240 |
| Chromosome: | chromosome 8 |
| Location: | 4392602 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g381950 | YAK1,TAR1 | (1 of 1) K18670 - dual specificity protein kinase YAK1 [EC:2.7.12.1] (YAK1); DYRK-type protein kinase/Yet-another-kinase 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGGTCGGGTGGGGGGGAGTGGGACGGCG |
| Internal bar code: | CAGGGGTAGTGATCTGGAGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 298 |
| LEAP-Seq percent confirming: | 93.2584 |
| LEAP-Seq n confirming: | 166 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTAGCCTGGACGGCTGTA |
| Suggested primer 2: | CGGAACAGGAAGAAGTCCAC |