Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.123268 |
Chromosome: | chromosome_17 |
Location: | 1621680 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g708000 | FAP260 | Flagellar Associated Protein, membrane-associated | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCAACCATTTGATGGCAGACATGGCTGAGC |
Internal bar code: | TTATTCTATAACCCTGGCCTAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 54 |
LEAP-Seq percent confirming: | 98.1818 |
LEAP-Seq n confirming: | 108 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GACTTCTTCTTGTGCAGGGC |
Suggested primer 2: | ACAACTCGTGCTGTGCTTTG |