| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.123282 |
| Chromosome: | chromosome 9 |
| Location: | 5652797 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401182 | CGL153 | predicted protein; (1 of 1) K14575 - AAA family ATPase (AFG2, DRG1, SPATA5) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATGTAATGTGGTGCTTATGTGACGAATG |
| Internal bar code: | ATGATATTCTCAGTATCAGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 766 |
| LEAP-Seq percent confirming: | 99.2173 |
| LEAP-Seq n confirming: | 1648 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCGGCTGTATGTGTGATG |
| Suggested primer 2: | CCTATCCTTCCCAGCCTTTC |