| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.123328 |
| Chromosome: | chromosome 4 |
| Location: | 3992792 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g231908 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTGCTGCGGGGCGGCCCACCATCATTAC |
| Internal bar code: | TACAGCCAGTACGTGAGTGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 413 |
| LEAP-Seq percent confirming: | 97.9687 |
| LEAP-Seq n confirming: | 5691 |
| LEAP-Seq n nonconfirming: | 118 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTCATGAGCAGCAGGCTA |
| Suggested primer 2: | GCAAGATCCACACACACACC |