Insertion junction: LMJ.RY0402.123377_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.123377
Chromosome:chromosome 2
Location:1392716
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g083350 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGTCACACAATGCACCCCCACACACACCA
Internal bar code:AGGCAGGATGGTTCGGGGTGACT

Confirmation - LEAP-Seq

LEAP-Seq distance:472
LEAP-Seq percent confirming:99.9153
LEAP-Seq n confirming:1180
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:TGATTCCTGATTCCTGGGAG
Suggested primer 2:CAAACATGTCTCTGCGCACT