| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.123413 |
| Chromosome: | chromosome 17 |
| Location: | 1557209 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g707500 | TOC120,TOC159 | Translocon at the outer envelope membrane of chloroplasts, receptor subunit; (1 of 1) PF11886 - Translocase of chloroplast 159/132, membrane anchor domain (TOC159_MAD) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGATGGGGGCTGGGGTAGCGACTGGGGG |
| Internal bar code: | GGCCGAGCACGGTACCAAGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 125 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 89 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGCCTCAGGGGTAAAGGT |
| Suggested primer 2: | AGTTTGGCTGGTTTGGTTTG |