| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.123439 |
| Chromosome: | chromosome 1 |
| Location: | 998856 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g005600 | FDX8 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGACCCCGCCCGGGTTATGTTGAGGCTG |
| Internal bar code: | AGTGGACAGCCATAAGTGATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 379 |
| LEAP-Seq percent confirming: | 99.6078 |
| LEAP-Seq n confirming: | 5333 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGGTGAGGAAGCACTAGG |
| Suggested primer 2: | CCTATCCATGGAAATCCCCT |