| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.123508 |
| Chromosome: | chromosome 9 |
| Location: | 5659300 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401256 | (1 of 13) PF05050 - Methyltransferase FkbM domain (Methyltransf_21) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTTTCCCCGTTTACCGCATGTGCCACCT |
| Internal bar code: | AGTAGAGGGTTAGGCAAGAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 755 |
| LEAP-Seq percent confirming: | 99.7944 |
| LEAP-Seq n confirming: | 1456 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGACAGCACCTCTGCCATA |
| Suggested primer 2: | AACTGCACACACGAGGAGTG |