Insertion junction: LMJ.RY0402.123547_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.123547
Chromosome:chromosome 1
Location:5514397
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g038950 SELF1,SELENOF SelF/SelM homolog; (1 of 1) PTHR13077:SF6 - 15 KDA SELENOPROTEIN CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGTTGTCAATGCGCACTGTCTCGCTGGGC
Internal bar code:CAACAATTCACGGCGAGGGAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:653
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:29
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GAATGTGGGAGGATGCTTGT
Suggested primer 2:GGTGATGTACTGATGTGCGG