Insertion junction: LMJ.RY0402.123589_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.123589
Chromosome:chromosome 10
Location:2395691
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g435350 ESU1,ESU1a Endosulfine cAMP-regulated phosphoprotein; (1 of 2) PF04667 - cAMP-regulated phosphoprotein/endosulfine conserved region (Endosulfine) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGAGTCAGGGAACCGCGGTCCCATCCCGAC
Internal bar code:CCGGAACGTATTGCAAGAGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:859
LEAP-Seq percent confirming:99.8982
LEAP-Seq n confirming:2943
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:TTTGCTATCAGTGCGTCCAG
Suggested primer 2:AGAAGGTCGCTGAAGGTTGA