| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.123605 |
| Chromosome: | chromosome 17 |
| Location: | 5489238 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g737351 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGACCAATGGGGGGTTTGGGGTCAGCAAT |
| Internal bar code: | ACGATGGCCGCCTTCCCAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 542 |
| LEAP-Seq percent confirming: | 99.6685 |
| LEAP-Seq n confirming: | 902 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTAAACCCAGCCACAATC |
| Suggested primer 2: | TAGCTCGAATACGCACATCG |