Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.123619 |
Chromosome: | chromosome 17 |
Location: | 1931145 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre17.g710600 | (1 of 112) 2.7.11.25 - Mitogen-activated protein kinase kinase kinase / MLTK; Protein tyrosine kinase | gene_edge/mRNA_edge/5'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAACCCGCGCGCTTTGCCGTGCCGTATAA |
Internal bar code: | CCAGATCTTCACGAGATGGCGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 709 |
LEAP-Seq percent confirming: | 99.6711 |
LEAP-Seq n confirming: | 2121 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTTCACAGCTACCCCTCAGC |
Suggested primer 2: | TACTGCTGCTACTGCCTTCG |