| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.123638 |
| Chromosome: | chromosome 5 |
| Location: | 2215731 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234655 | (1 of 1) K17550 - protein phosphatase 1 regulatory subunit 7 (PPP1R7, SDS22) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGGAGGGGCGTAAGCATTGTGTGTGTGG |
| Internal bar code: | GGACGGGTGGTCGGTTGTTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 814 |
| LEAP-Seq percent confirming: | 99.7635 |
| LEAP-Seq n confirming: | 7594 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATAATGATTGGGTTTGGG |
| Suggested primer 2: | GCAGACGGGACAACAAAAAT |