| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.123682 |
| Chromosome: | chromosome 4 |
| Location: | 1172278 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g215700 | (1 of 4) IPR009030//IPR011641 - Insulin-like growth factor binding protein, N-terminal // Tyrosine-protein kinase ephrin type A/B receptor-like | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTTGCACTTACTTTGAGTGTGACTGTGT |
| Internal bar code: | CAAGTGATTGTGCAACTGCAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 307 |
| LEAP-Seq percent confirming: | 99.2993 |
| LEAP-Seq n confirming: | 4960 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCGTACGTAACTGCCAGC |
| Suggested primer 2: | CACTCACTCCAGCTGAACCA |