| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.123689 |
| Chromosome: | chromosome_1 |
| Location: | 915859 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g004900 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATTTGCATTATTGCCACCAGCTGTGACAAG |
| Internal bar code: | GACGACGCTCTGAAGTCCTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 898 |
| LEAP-Seq percent confirming: | 98.0152 |
| LEAP-Seq n confirming: | 1679 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTCTCCACCTCTGTCAAG |
| Suggested primer 2: | GTGGTGGGACAAGCTCGTAT |