Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.123691 |
Chromosome: | chromosome_3 |
Location: | 5071269 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g181100 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GAACCACAGCTGGTGGGGCGACTGTACCAA |
Internal bar code: | GACCGGGTTCAGGGGGCCAAGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 883 |
LEAP-Seq percent confirming: | 92.0308 |
LEAP-Seq n confirming: | 2991 |
LEAP-Seq n nonconfirming: | 259 |
LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACCTTCTCCGAAGCTCAACA |
Suggested primer 2: | CATGTTTGTATGTGGCCGAG |