| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.123693 |
| Chromosome: | chromosome 12 |
| Location: | 3482118 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g496150 | (1 of 1) PTHR20953//PTHR20953:SF4 - KINASE-RELATED // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCGCCTGCGGAAGGGGTGCGATGGCGGG |
| Internal bar code: | ATGATCTTCAGAATAACGCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 583 |
| LEAP-Seq percent confirming: | 99.6859 |
| LEAP-Seq n confirming: | 3174 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAGCCTGCTCTTGACTCC |
| Suggested primer 2: | GCAAGAGTGGGCAGAGAAAC |