| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.123693 |
| Chromosome: | chromosome 12 |
| Location: | 3482122 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g496150 | (1 of 1) PTHR20953//PTHR20953:SF4 - KINASE-RELATED // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGCTGCTGCTGGGGCGGCCAGGTGTGGG |
| Internal bar code: | GTGGGCCGCCGAGGCCTATTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 786 |
| LEAP-Seq percent confirming: | 97.7396 |
| LEAP-Seq n confirming: | 1989 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAGCCTGCTCTTGACTCC |
| Suggested primer 2: | GCAAGAGTGGGCAGAGAAAC |