Insertion junction: LMJ.RY0402.123720_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.123720
Chromosome:chromosome 2
Location:2718435
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g093600 TEH3 (1 of 6) PF03061 - Thioesterase superfamily (4HBT); Thioesterase-like protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACCCAGATACCCCGCTTAACCATAACCCT
Internal bar code:ACTCCCCGCGAACGCAGCATGA

Confirmation - LEAP-Seq

LEAP-Seq distance:917
LEAP-Seq percent confirming:99.7118
LEAP-Seq n confirming:1384
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTGTTGCCCATGTCACTC
Suggested primer 2:GCATGCATTACCAATTGTGC