| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.123724 |
| Chromosome: | chromosome 10 |
| Location: | 3347679 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g443850 | (1 of 1) PTHR11851//PTHR11851:SF136 - METALLOPROTEASE // PROTEIN YHJJ | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGACTGTATGGAGTTACATTTAGGATCAT |
| Internal bar code: | ACGTCAACCGTGGTACATAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 296 |
| LEAP-Seq percent confirming: | 99.759 |
| LEAP-Seq n confirming: | 9934 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGATAGCACACCTCTGCCC |
| Suggested primer 2: | ACCTGACTCTCACCCACCAC |