| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.123725 |
| Chromosome: | chromosome 6 |
| Location: | 8916320 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g311250 | (1 of 3) PTHR23213//PTHR23213:SF214 - FORMIN-RELATED // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTAGCCCCGCTACCAATGCTTTTTTGCGC |
| Internal bar code: | TGTACTGTCGAGACGCGGGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 89 |
| LEAP-Seq percent confirming: | 99.422 |
| LEAP-Seq n confirming: | 344 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGAACTTGGGTGATAAGGC |
| Suggested primer 2: | GCGGCTGAGTACAGTCCTTC |