| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.123725 |
| Chromosome: | chromosome 12 |
| Location: | 1491309 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g487450 | (1 of 1) PTHR28654:SF1 - AXIN INTERACTOR, DORSALIZATION-ASSOCIATED PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGAGTGTCCTCCATTTATATCACTTCGTC |
| Internal bar code: | CAAGGGAAGACTTGCATAGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 343 |
| LEAP-Seq percent confirming: | 99.7439 |
| LEAP-Seq n confirming: | 779 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGCGCACCATAAGAGCAT |
| Suggested primer 2: | TCACCTAGCCAGCTGTGATG |