Insertion junction: LMJ.RY0402.123741_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.123741
Chromosome:chromosome 6
Location:7687753
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g301802 3'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGAGCTGCCGAATTTATGTTTCCCCAGCC
Internal bar code:TGGTCGGACGTGTGCGACGGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:627
LEAP-Seq percent confirming:99.7983
LEAP-Seq n confirming:3463
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GGATTCACCATGGGTTTACG
Suggested primer 2:CCGTACCCGTACTGTGGAGT