Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.123746 |
Chromosome: | chromosome_9 |
Location: | 6976705 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g410700 | MDY5,MDH5 | NADP-dependent malate dehydrogenase, chloroplastic | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTATGAGGTGTTCGGCGACGCCGACTGGGC |
Internal bar code: | GAGTGACGTGCAGAGAAGATCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 552 |
LEAP-Seq percent confirming: | 99.8682 |
LEAP-Seq n confirming: | 2274 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAGTACCCAGCAAACATGGC |
Suggested primer 2: | CAGGGACTCGTGTCCAATCT |