Insertion junction: LMJ.RY0402.123748_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.123748
Chromosome:chromosome 17
Location:5728848
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre17.g739050 (1 of 230) IPR017986 - WD40-repeat-containing domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCACCCGGTCCCGGCACTGCCGCCGCCGC
Internal bar code:TTTGTGCTATTTCGTACGATCG

Confirmation - LEAP-Seq

LEAP-Seq distance:3
LEAP-Seq percent confirming:70.3641
LEAP-Seq n confirming:1237
LEAP-Seq n nonconfirming:521
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CTACAGCCGCTTTCTCCAAC
Suggested primer 2:AGGTGGATGAGTTCCACGAG