Insertion junction: LMJ.RY0402.123775_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.123775
Chromosome:chromosome 2
Location:1897755
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre02.g087666 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTTCTCTTGTCCGCCATGCATCCAATCAT
Internal bar code:GCAGGGGAGGCTTCGACGACAA

Confirmation - LEAP-Seq

LEAP-Seq distance:793
LEAP-Seq percent confirming:54.9528
LEAP-Seq n confirming:233
LEAP-Seq n nonconfirming:191
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:ACACACTCCCTCACTGACCC
Suggested primer 2:ACCATCACGTTCTCCTGACC