Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.123783 |
Chromosome: | chromosome_10 |
Location: | 4258101 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g450450 | FAP18 | Flagellar Associated Protein, serine endopeptidase | antisense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTTCATATGTCGTTCTGGCGGTGTTTGCAA |
Internal bar code: | GGGGCTCGGCGGACTTTGGGGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 670 |
LEAP-Seq percent confirming: | 99.7379 |
LEAP-Seq n confirming: | 2283 |
LEAP-Seq n nonconfirming: | 6 |
LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACTTGACCGATGTGCTACCC |
Suggested primer 2: | GGCACATGAACTTTGAGGGT |