Insertion junction: LMJ.RY0402.123838_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.123838
Chromosome:chromosome 1
Location:5450503
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g038350 (1 of 39) IPR009003 - Peptidase S1, PA clan intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCATCTTTGCCGAAAGCTTCCGAACGTCT
Internal bar code:ATGATGATTGGAGAGACGGGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:131
LEAP-Seq percent confirming:99.122
LEAP-Seq n confirming:4516
LEAP-Seq n nonconfirming:40
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:GAGCAGGAAGCTCCACAAAC
Suggested primer 2:CTACGGGCACGGACACTATT