Insertion junction: LMJ.RY0402.123844_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.123844
Chromosome:chromosome 7
Location:2027517
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g325761 (1 of 2) IPR000104//IPR003439//IPR003593//IPR011527//IPR027417 - Antifreeze protein, type I // ABC transporter-like // AAA+ ATPase domain // ABC transporter type 1, transmembrane domain // P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATAGTGTGTTGTGCGCTTTGCGCTGTGCG
Internal bar code:TACCCGCACATTGCTGCGGCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:249
LEAP-Seq percent confirming:99.8187
LEAP-Seq n confirming:1652
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTTGGGTTCTGGAATGAA
Suggested primer 2:TACACCCACAGCCACCTGTA